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Histones and other DNA-binding proteins play an important role in chromosome organization, gene regulation and post-translational modification. Core components of histones can be covalently modified through methylation, acetylation, phosphorylation and sumoylation. These modifications are essential for gene expression, regulation, DNA repair and chromosome condensation. Minute™ Histone/DNA Binding Protein Extraction Kit is designed to efficiently extract histones and other DNA-binding proteins from cultured cells and isolated cells. Unlike many other commercial histone extraction kits that employ acid or high-salt extraction methods, Minute™ Histone/DNA Binding Protein Extraction Kit is efficient in extracting histones and other DNA-binding proteins that can be used for internal controls for histone modification analysis. In comparison, acid extraction protocol that selectively extracts basic proteins and a good internal control is usually not possible. Minute™ Histone/DNA Binding Protein Extraction Kit can extract histones from 0.5 to 5 million cells in less than 10 minutes with high protein yield (1-2.5 mg/ml), making it the fastest and most robust histone extraction kit available on the market.
Kit includes:
| Items | Quantity |
| Buffer A | 25 ml |
| Buffer B | 25 ml |
| Filter Cartridges | 50 units |
| Collection Tubes with Caps | 50 units |
References (1)
1. Zhao, X., Xue, X., Wang, J., Dai, S., Peng, C., & Li, Y. (2022). Quercetin alleviates ethanol‐induced hepatic steatosis in L02 cells by activating TFEB translocation to compensate for inadequate autophagy. Phytotherapy Research.
